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mlui digested plenti x1 hygro mcherry ramp4 vector  (Addgene inc)


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    Structured Review

    Addgene inc mlui digested plenti x1 hygro mcherry ramp4 vector
    Mlui Digested Plenti X1 Hygro Mcherry Ramp4 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mlui+digested+plenti+x1+hygro+mcherry+ramp4+vector/pLenti-X1-hygro-mCherry-RAMP4+(Plasmid+%23118391)/pm33870132-452-11-20
    Average 92 stars, based on 6 article reviews
    mlui digested plenti x1 hygro mcherry ramp4 vector - by Bioz Stars, 2026-09
    92/100 stars

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    Transfection:

    Article Title: ATG9A regulates proteostasis through reticulophagy receptors FAM134B and SEC62 and folding chaperones CALR and HSPB1.
    Article Snippet: Generation of EATR, CCER, and mCherry-ER-3 stable lines To generate the EATR stable line, HEK293 cells were transfected with SspI digested TetOn-mCherry-eGFP-RAMP4 vector (gift from Jacob Corn; Addgene plasmid #109014) and selected in DMEM-10 with 400ug/mL G418 (Invitrogen #10131027). .. To generate the CCER stable line, HEK293 cells were transfected with MluI digested pLenti-X1-hygro-mCherry- RAMP4 vector (gift from Jacob Corn; Addgene plasmid #118391) and selected in DMEM-10 + 100ug/mL Hygromyocin B (Invitrogen #10687010). .. To generate the mCherry-ER-3 stable line, HEK293 cells were transfected with undigested mCherry-ER-3 vector (gift from Michael Davidson; Addgene plasmid #55041) and selected in DMEM-10 with 400ug/mL G418.

    Plasmid Preparation:

    Article Title: ATG9A regulates proteostasis through reticulophagy receptors FAM134B and SEC62 and folding chaperones CALR and HSPB1.
    Article Snippet: Generation of EATR, CCER, and mCherry-ER-3 stable lines To generate the EATR stable line, HEK293 cells were transfected with SspI digested TetOn-mCherry-eGFP-RAMP4 vector (gift from Jacob Corn; Addgene plasmid #109014) and selected in DMEM-10 with 400ug/mL G418 (Invitrogen #10131027). .. To generate the CCER stable line, HEK293 cells were transfected with MluI digested pLenti-X1-hygro-mCherry- RAMP4 vector (gift from Jacob Corn; Addgene plasmid #118391) and selected in DMEM-10 + 100ug/mL Hygromyocin B (Invitrogen #10687010). .. To generate the mCherry-ER-3 stable line, HEK293 cells were transfected with undigested mCherry-ER-3 vector (gift from Michael Davidson; Addgene plasmid #55041) and selected in DMEM-10 with 400ug/mL G418.



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